Comparative Sequence Analysis

E R R _ W I S E Detection of Frameshift Sequencing Errors

Note on Usage

The default values and scalings provided by the program are to be treated as suggestions and more than likely they will not be optimal in a given case.

Optimal penalties depend on the actual score values for the aligned sequences. Thus they are affected by divergence of the sequences, residue biases, choice of residue exchange matrix and so on. This means that the gap and frame penalties cannot be defined a priori and need to be calibrated by trial and error.

See the Help on choosing Parameters for an overview or read the following publication for all the details:

Birney, E., Thompson, J.D. and Gibson, T.J. (1996) Nucleic Acids Res., 24, 2730-2739

Information on the complete set of tools can be found at the WiseTools HomePage.

Now just fill in the fill-out form and hit the submit button to start processing.

The Penalties

Gap Penalty [0 - 10000] Gap Extension Penalty [0 - 10000] Frame Shift Penalty [0 - 10000] Frame Extension Penalty [0 - 10000] Stop Codon Penalty [0 - 10000] Show Forward Strands YES NO Show Backward Strands YES NO Residue Exchange Matrix Paste in your DNA Sequence in FASTA or GCG format: Paste in your Protein Sequence in FASTA or GCG format:

Last modified on 12.Oct.2001 (prev. Feb. 6, 1998)